Journal: Frontiers in Microbiology
Article Title: Irisin alleviated the reproductive endocrinal disorders of PCOS mice accompanied by changes in gut microbiota and metabolomic characteristics
doi: 10.3389/fmicb.2024.1373077
Figure Lengend Snippet: The effect of r-irisin on the expression of follicle development and atresia-related proteins in vivo. (A) Immunostaining of cleaved caspase-3 in ovary sections from each group of mice. (B) Immunostaining of GDF-9 in ovary sections from each group of mice. (C) Immunostaining of the granulosa cell-oocyte bridge protein CX37/GJA4 in ovary sections from each group of mice. (D) Immunostaining of CYP19a1 in ovary sections from each group of mice. The bar charts in the right column were the immunohistochemical semiquantitation depending on average light intensity. Sample size: n = 7 (7 mice in each group). Statistical method: ANOVA. Significance: * p < 0.05, ** p < 0.01, and **** p < 0.0001.
Article Snippet: The primary antibodies used here were as follows: anti-cleaved-caspase3 (1:400, 9664, CST), anti-GDF-9(1:2000, ab254323, abcam), anti-CX37/GJA4 (0.5–1 μg/mL, ab254323, abcam), and anti-P450 aromatase (Cyp19a1) antibody (1:200, ab18995, abcam).
Techniques: Expressing, In Vivo, Immunostaining, Immunohistochemical staining